RESEARCH SCIENTIST/ENGINEER 1

Req #: 238197Department: IMMUNOLOGYPosting Date: 08/26/2024Closing Info:

Closes On 09/02/2024Salary: $3,750 - $4,167 per monthLimited Recruitment: Open to UW Employees onlyShift: First ShiftNotes:

As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701a11y.pdf )As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world.UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty.The Immunology Department at the University of Washington School of Medicine has an outstanding opportunity for a Research Scientist/Engineer 1 in the Savan laboratory. POSITION PURPOSE Research in the Savan laboratory is focused on understanding human genetic variations and RNA-regulatory mechanisms to gain insights into the heterogeneity in immune responses against viral infections. The main interest is to identify functional variants found in the coding and non-coding regions that determine susceptibility or resistance to life-threatening viral infections.DUTIES AND RESPONSIBILITIES 20% - Prepare laboratory reagents, conduct Inventory, order, and restock laboratory supplies.

10% - Drafting Biological Use Authorization approvals for BSL1, BSL2, and BSL2+ pathogens and preparing the lab for inspections for BSL1, BSL2, and BSL2+ spaces.

10% - Onboarding research personal, training new and existing personal on safety protocols, including biological and chemical safety.

10% - Basic molecular biology including miRNA extraction and quantification, mutagenesis, luciferase assays, transfection of plasmids/siRNAs/miRNA, nucleofection, western blotting, plasmid cloning, and qRT-PCR.

10% - Multicolor flow cytometry, image-stream, and immunofluorescence microscopy.

10% - Perform genomic techniques such as ChIP seq, ATAC seq, and RNA-seq library preps.

10% - Organize research data.

10% - Guides the work of undergraduate employees.

5% - Participates in laboratory meetings.

5% - Perform related duties as required.MINIMUM REQUIREMENTS Bachelor’s degree in biological sciences or related field with one year of experience with molecular biology techniques including miRNA extraction and quantification, mutagenesis, luciferase assays, transfection of plasmids/siRNAs/miRNA, nucleofection, western blotting, plasmid cloning, and qRT-PCR, or equivalent combination of education and experience. Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. Additional Requirements:

Experience in genomic techniques such as ChIP seq, ATAC seq, and RNA-seq.

Well versed in flow-cytometry and image stream assays and subsequent downstream analyses.

Experience with Microsoft Office Excel for data reporting and experimental calculations.

Must achieve deadlines and specified project milestones in an efficient manner.

Highly motivated and able to work in a team environment within a laboratory setting. DESIRED QUALIFICATIONS

Two years of experience with molecular biology techniques including miRNA extraction and quantification, mutagenesis, luciferase assays, transfection of plasmids/siRNAs/miRNA, nucleofection, western blotting, plasmid cloning, and qRT-PCR. Application Process: The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.